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Future Dental Journal

Abstract

Purpose: This study aimed to evaluate the potential ameliorating effect of bone marrow derived mesenchymal stem cells (BM-MSCs) on tongue papillae of rats with experimentally induced hypothyroidism using environmental scanning electron microscope (ESEM) and fluorescent microscopic evaluation. Material and Methods: For this study 30 adult male albino rats were divided into three groups (ten rats each): (1) Control group: The rats allowed to access freely balanced diet and freshwater supply during the course of the experimental period (8 weeks). (2) Hypothyroidism group: To induce hypothyroidism the rats received carbimazole dissolved in distilled water (5 mg/250g body weight/day) for 35 days. (3) BM-MSC treated group: The rats received carbimazole for 35 days and after confirmation of hypothyroidism the rats received a single intravenous injection of BM-MSCs (1× 106 cells) via tail vein. At the end of the experiment, all rats were sacrificed; tongue was dissected and prepared for fluorescent and scanning electron microscopic evaluation. Results: Tongue papillae of hypothyroidism group showed marked morphological alterations, unusual variable orientation, loss of the regular hornified keratin coverage and atrophied papillae with apparent wide inter-papillary regions and atrophy of gustatory pits. The tongue papillae in BM-MSCs treated group revealed apparent improvement in their architecture. Conclusion: Hypothyroidism has degenerative effects on tongue papillae. BM-MSCs administration reduces the damaging effects of hypothyroidism on the papillae.

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