Activity Performance and Stability of Functionalized SBA -15 Mesoporous Silica for Lipase Immobilization

Authors

Ahmed Mubarak Alsobaai Dr

Abstract

SBA -15 Mesoporous Silica was synthesized and functionalized with aminopropyl-, phenyl- and chloropropyl- using post-synthesis method. The esterification of citronellol with lauric acid in batch mode at 37 ◦C was used to measure the performance of the immobilized enzymes. Characterization of the supports revealed the satisfactory development of mesoporosity with uniform internal channels distribution. Pure SBA-15 showed the typical hexagonal pore and straight channels while the functionalized SBA-15 with triethoxysilane caused pore size to decrease and the distortion of internal channels occurred to subsequently improved the degree of immobilization. The best support among the four was the 3-aminotriethoxysilane functionalized SBA-15 that showed about 77 % conversion after 24 h. The stability of lipase immobilized on 3-aminotriethoxysilane functionalized mesoporous SBA-15 was also investigated. It was found that the immobilized enzyme was stable with minimal leaching. Repetition of reaction using the same immobilized enzyme also showed promising result with nearly 60 % acid conversion after 3 cycles.

Recommended Citation

Alsobaai, Ahmed Mubarak Dr (2017) "Activity Performance and Stability of Functionalized SBA -15 Mesoporous Silica for Lipase Immobilization," Hadhramout University Journal of Natural & Applied Sciences: Vol. 14: Iss. 1, Article 5.
Available at: https://digitalcommons.aaru.edu.jo/huj_nas/vol14/iss1/5