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Hebron University Research Journal-A (Natural Sciences) - (مجلة جامعة الخليل للبحوث- أ (العلوم الطبيعيه

Hebron University Research Journal-A (Natural Sciences) - (مجلة جامعة الخليل للبحوث- أ (العلوم الطبيعيه

Abstract


The fungus Botrytis cinerea grown on shaking potato dextrose broth (PDB) medium amended with 10 mM methionine produced ethylene. Ethyleneproduction reached a peak after 4 days of incubation; however, there was a high variability in production between the isolates. The range of ethylene produced by twenty four isolates of the fungus was from 383 to 6789 µl/g/h with average production of 2091 µl/g/h. The results showed considerable intraspesific variation in ethylene production of B. cinerea.

The role of exogenously applied ethylene (Ethephon® ) in the interaction between B. cinerea and tomato (Lycopersicon esculentum) and bean (Phaseolus vulgaris) plants was studied in vitro and in vivo. Ethephon® has not significantly affected the fungus mycelium growth rate on potato dextrose agar (PDA), while it significantly increased disease severity by 50.4 % - 55.8 % on tomato plants and by 27.6 %-33.8 % on bean plants at concentration 200-600 µg/ml. Aminoethoxyvinylglycine (AVG) significantly decreased mycelium growth in vitro at 200 µg/ml by 47 %, and it decreased disease severity by 45 %- 49 % at concentrations 200 - 300 µg/ml on tomato plants and 55 % -75 % at 100-300 µg/ml on bean plants.

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