Hebron University Research Journal-A (Natural Sciences) - (مجلة جامعة الخليل للبحوث- أ (العلوم الطبيعيه
Recent advances in biochemical and genetic studies point to the steady-state production of hydrogen peroxide (H2O2) in plant tissues. H2O2 was traditionally viewed as a harmful by-product of reactive oxygen species (ROS) produced during plant metabolism. This view has, however, been changed by mounting evidence for its involvement in biotic and abiotic stress and its role in signal transduction in plants. During stress, such as following pathogen infection and wounding, plants adjust their cellular metabolism and the redox (reduction/oxidation) status of the cell resulting in increased intercellular levels of H2O2. Due to H2O2 toxicity, plants use antioxidants to keep its production under tight control and an abrupt increase in its levels results in a serious imbalance between H2O2 and antioxidant levels that eventually leads to cell death. Increased interest in determining the redox status of stressed plants has led to the development of several methods for H2O2 detection. Levels of H2O2 measured from a variety of plant species show a wide range (e.g., nM to mM) of H2O2 concentrations, a clear indication of technical difficulties in quantifying H2O2 levels. In this brief review, several of the methods used to detect H2O2 levels are discussed, particularly those that are used for quantitative estimations of H2O2 based on peroxidase-mediated assays. Because of various problems associated with H2O2 detection in plants, it is recommended that several methods be used to verify H2O2 contents before any interpretation of data is made
"An Overview of Hydrogen Peroxide Production and Cellular Determination in Plants,"
Hebron University Research Journal-A (Natural Sciences) - (مجلة جامعة الخليل للبحوث- أ (العلوم الطبيعيه: Vol. 3:
2, Article 7.
Available at: https://digitalcommons.aaru.edu.jo/hujr_a/vol3/iss2/7