Applicability of Fibrous cellulose –D in extracting double-stranded RNA (dsRNA) of ToBRFV

Authors

Raed Alkow, Biology & Biotechnology Department; Faculty of Science, An-Najah National University, Nablus, Palestine National Agricultural Research Center (NARC), Jenin, PalestineFollow
Reem Hijaz, Biology & Biotechnology Department; Faculty of Science, An-Najah National University, Nablus, Palestine National Agricultural Research Center (NARC), Jenin, PalestineFollow
Beesan Jamoos, Biology & Biotechnology Department; Faculty of Science, An-Najah National University, Nablus, Palestine National Agricultural Research Center (NARC), Jenin, PalestineFollow
Najah Khandaqji, Biology & Biotechnology Department; Faculty of Science, An-Najah National University, Nablus, Palestine National Agricultural Research Center (NARC), Jenin, PalestineFollow
We’am Daraghmeh, Biology & Biotechnology Department; Faculty of Science, An-Najah National University, Nablus, Palestine National Agricultural Research Center (NARC), Jenin, PalestineFollow
Saja Salah, Biology & Biotechnology Department; Faculty of Science, An-Najah National University, Nablus, Palestine National Agricultural Research Center (NARC), Jenin, PalestineFollow
Alaa Bari, Biology & Biotechnology Department; Faculty of Science, An-Najah National University, Nablus, Palestine National Agricultural Research Center (NARC), Jenin, PalestineFollow
Rahaf Ben Ali, Biology & Biotechnology Department; Faculty of Science, An-Najah National University, Nablus, Palestine National Agricultural Research Center (NARC), Jenin, PalestineFollow
Osama Alabdalla, Biology & Biotechnology Department; Faculty of Science, An-Najah National University, Nablus, Palestine National Agricultural Research Center (NARC), Jenin, PalestineFollow

Abstract

The viral replicative form double strand RNA (dsRNA) is a unique feature to the plant RNA viruses that used for non-specific detection of plant viruses. For decades; fibrous cellulose -11 (CF11) was used for dsRNA purification, but recently was no-more produced. Other fibrous celluloses (CF-A; -B; -C; -D; and –E) were suggested as alternatives to CF11. This study was to evaluate the applicability and reproducibility of using CF-D with two different dsRNA extracting methods: micro-spin column and modified Dodds extraction methods. The best results were recorded on Dodds extraction method over micro-spin one. This results also confirmed the applicability of extracting dsRNAs from infected plant tissues with CF-D as an alternative to CF-11. Interestingly, the sample which was exhibiting Tomato brown rugose fruit virus (ToBRFV) symptoms but negatively tested by reverse transcription-plymerase chain reaction (RT-PCR); were exhibited band (~6000 bp) approximate size of the suspected ToBRFV. Thus, dsRNA emphasizing its applicability in detecting the RNA variants of the virus. From the best of our knowledge, this research was the first to be conducted and recommending using CF-D in modified Dodds extraction method. Moreover, research on applying CF-D for RNA extraction would be highly recommended.

Recommended Citation

Alkow, Raed; Hijaz, Reem; Jamoos, Beesan; Khandaqji, Najah; Daraghmeh, We’am; Salah, Saja; Bari, Alaa; Ben Ali, Rahaf; and Alabdalla, Osama (2023) "Applicability of Fibrous cellulose –D in extracting double-stranded RNA (dsRNA) of ToBRFV," Jerash for Research and Studies Journal مجلة جرش للبحوث والدراسات: Vol. 24: Iss. 2, Article 18.
Available at: https://digitalcommons.aaru.edu.jo/jpu/vol24/iss2/18