Isolation of Proteins That Have the ‎Ability to Inhibit Potato Y Virus ‎‎(PVY) Replication from Datura ‎Plants - Jimson Weed Datura ‎Strmonium

Authors

Raqeeb Al-Ani, Bagdad University, Iraq‎Follow
Osama Al-Issawi, Bagdad University, Iraq‎Follow
Shaalan Al-‎Mashaikhi, Bagdad University, Iraq‎Follow

Abstract

Two protein portions from Datura inhibiting the multiplication of PVY “were obtained by ‎filtration through sephadex G - 100. The first was maintained its activity at 60 с for 10 min; ‎whereas the other lost its activity at this temp. The SDS- polyacrylamide gel electrophoresis of ‎the heat resistant one revealed the presence of 8 polypeptides with molecular weights; 12000 ‎‎, 13000 , 22000 , 25000 , 29000 , 40000 and two other of smaller molecular weights . No ‎serological reaction was ovserced between these proteins and the antiserum of PVA, PVS, ‎PVM, PVX and PVY. No precipitate was observed when the virus was mixed with a ‎suspension of these proteins which inيindicated that these proteins were unable to ‎precipitate the virus. It was found that the treatment of the plants by a suspension of the ‎stable proteins 24 hours before the inoculation with PVY or mixed with the virus at the ‎moment of the inoculation leads to prevention of the infection. Whereas, the treatment of ‎the plants 24 hours after the inoculation with PVY leads to delay of the development of ‎infection 20 days compared to 13 days with the virus alone. Result showed that these proteins ‎possess the capacity of inhibiting the infection before the entrance of PVY into the tissue, but ‎not after its multiplication

Recommended Citation

Al-Ani, Raqeeb; Al-Issawi, Osama; and Al-‎Mashaikhi, Shaalan (2006) "Isolation of Proteins That Have the ‎Ability to Inhibit Potato Y Virus ‎‎(PVY) Replication from Datura ‎Plants - Jimson Weed Datura ‎Strmonium," Jerash for Research and Studies Journal مجلة جرش للبحوث والدراسات: Vol. 7: Iss. 1, Article 1.
Available at: https://digitalcommons.aaru.edu.jo/jpu/vol7/iss1/1