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Delta University Scientific Journal

Delta University Scientific Journal

Abstract

Aims: The primary aim of this study was to compare the effects of human platelet rich fibrin (hPRF) exudate Vs fetal bovine serum (FBS) on the proliferation and osteodifferentiation of human dental pulp stem cells (hDPSCs). The secondary one was to determine the optimum concentration of hPRF exudate inducing hDPSCs proliferation and osteodifferentiation. Study design: One hundred and forty ml of blood was collected from 8 healthy donors. hPRF exudate was prepared by the direct method. hDPSCs were isolated from impacted mandibular third molars of twelve donors by the outgrowth method. For cell viability and proliferation rate testing, 96 well plates were used and the assay was done in duplicate and the trial repeated four times under the same conditions. Six wells were used to contain 10% FBS, serum free media, 1%, 5%, 10% and 20% concentrations of hPRF exudates, respectively. The proliferation assay was carried out after 1 and 3 days by MTS tetrazolium cell proliferation assay kit and Elisa reader. The study design for osteodifferentiation protocol was exactly as the proliferation one and instead the assay was carried out after 7 days by alizarin red with Elisa reader. Results: Compared to 10%FBS, 10% hPRF exudate was the optimum concentration for hDPSCs proliferation, while 1% hPRF exudate was the optimum concentration for osteodifferentiation of hDPSCs. Conclusion: The preparation technique of hPRF exudate is simpler and cheaper than that of FBS. Avoiding the risk of zoonosis which may be occurred with FBS, it is recommended to use 10% hPRF exudate for proliferation and 1% for osteodifferentiation.

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