Future Dental Journal


Background: Passage of gingival fibroblasts/gingival mesenchymal stem cells through macro-perforated barrier membranes may allow them to contribute positively in periodontal regeneration. The present study was designed to evaluate the effect of use of Leucocyte Platelet Rich Fibrin (L-PRF) on periosteum-derived stem/ progenitor cells (PDPCs) and gingival mesenchymal stem cells (GMSCs) encouraging their passage through membrane perforations. Methods: twenty patients were randomly allocated into two equal groups; Group I perforated membrane (PM): included intrabony defects treated by perforated membranes. Group II Leucocyte-platelet rich fibrin + perforated membrane (L-PRF + PM): included intrabony defects treated by Leucocyte platelet rich fibrin & perforated membranes.Clinical and radiographic parameters were taken at baseline and 6 months after therapy. Results: there was a statistically significant difference in each group between baseline and 6 month follow-up intervals regarding probing depth (PD), clinical attachment level (CAL) gain and defect basal level (DBL), while there was no statistically significant difference in change of PD, CAL, DBL between both groups where (p=0.429), (p=0.972) and (p=0.13) respectively. Conclusion: perforated guided tissue membrane alone proved effective in improving the clinical outcomes for treating intrabony defects.