Abstract
Purpose: This study aimed to evaluate the potential ameliorating effect of bone marrow derived mesenchymal stem cells (BM-MSCs) on tongue papillae of rats with experimentally induced hypothyroidism using environmental scanning electron microscope (ESEM) and fluorescent microscopic evaluation. Material and Methods: For this study 30 adult male albino rats were divided into three groups (ten rats each): (1) Control group: The rats allowed to access freely balanced diet and freshwater supply during the course of the experimental period (8 weeks). (2) Hypothyroidism group: To induce hypothyroidism the rats received carbimazole dissolved in distilled water (5 mg/250g body weight/day) for 35 days. (3) BM-MSC treated group: The rats received carbimazole for 35 days and after confirmation of hypothyroidism the rats received a single intravenous injection of BM-MSCs (1× 106 cells) via tail vein. At the end of the experiment, all rats were sacrificed; tongue was dissected and prepared for fluorescent and scanning electron microscopic evaluation. Results: Tongue papillae of hypothyroidism group showed marked morphological alterations, unusual variable orientation, loss of the regular hornified keratin coverage and atrophied papillae with apparent wide inter-papillary regions and atrophy of gustatory pits. The tongue papillae in BM-MSCs treated group revealed apparent improvement in their architecture. Conclusion: Hypothyroidism has degenerative effects on tongue papillae. BM-MSCs administration reduces the damaging effects of hypothyroidism on the papillae.
Recommended Citation
Reyad A, Abdel Moneim R. The Potential Ameliorating Effect of Bone Marrow Derived Mesenchymal Stem Cells on the Tongue Papillae of Rats with Experimentally Induced Hypothyroidism. Future Dental Journal. 2021; 7(1). doi: https://doi.org/10.54623/fdj.7014.
DOI
https://doi.org/10.54623/fdj.7014